Production and characterization of psychrophilic α-amylase from a psychrophilic bacterium, Shewanella sp. ISTPL2
نویسندگان
چکیده
منابع مشابه
Multiple mutants of a psychrophilic α-amylase 1 Stepwise adaptations to low temperature as revealed by multiple mutants of a psychrophilic α-amylase from an Antarctic bacterium*
Background: Cold-adapted enzymes remain catalytically active at low temperatures. Results: Mutants of a cold-adapted alpha-amylase stabilized by engineered weak interactions and a disulfide bond have lost the kinetic optimization to low temperatures. Conclusion: The disappearance of stabilizing interactions in psychrophilic enzymes increases the dynamics of active site residues at low temperatu...
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In this study, a novel α-amylase was cloned from Bifidobacterium longum and named BiLA. The enzyme exhibited optimal activity at 20 °C and a pH value of 5.0. Kinetic analysis using various carbohydrate substrates revealed that BiLA had the highest k(cat/)K(m) value for amylose. Interestingly, analysis of the enzymatic reaction products demonstrated that BiLA specifically catalyzed the hydrolysi...
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A novel facultatively psychrophilic bacterium, strain S-1, which exhibits extraordinarily high catalase activity was isolated from the drain pool of a fish product processing plant that uses H2O2 as a bleaching and microbicidal agent. The catalase activity of the isolate was 1 or 2 orders of magnitude higher than those of Corynebacterium glutamicum, Staphylococcus aureus, Pseudomonas fluorescen...
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ژورنال
عنوان ژورنال: Amylase
سال: 2020
ISSN: 2450-9728
DOI: 10.1515/amylase-2020-0001